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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Efficiently generate functional hepatic cells from human pluripotent stem cells by complete small-molecule strategy

Fig. 5

Expansion and maintain of small-molecule-derived HBs. A A schematic description of stepwise differentiates hPSCs into HBs and HLCs. B Flow cytometric sorting of E-pCAM+ HBs from day 8 differentiated cells. C Phase-contrast images of expanding HBs. Scale bar 100 μm. D Flow cytometric analyses of E-pCAM and Ki67 expression in expanded HBs (passage 20). E Immunostaining analyses of HBs marker proteins on expanded HBs. Scale bars 100 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512