Fig. 5
From: Chemically defined and small molecules-based generation of sinoatrial node-like cells
Electrophysiological characterization of SANLCs generated under chemically defined conditions. a Representative action potential recording using whole-cell patch from non-purified CDM3 group, purified CDM3 group, non-purified PBC group, and purified PBC group. b Patch clamp recordings of induced cells from non-purified CDM3 group (20 cells), purified CDM3 group (20 cells), non-purified PBC group (12 cells), and purified PBC group (20 cells), demonstrating MDP, peak voltage, APA, APD90, APD50, and dV/dtmax (maximal rate of depolarization). c Bar graph indicates proportions of cardiomyocyte subtypes at day 32. MDP, maximum diastolic potential; APA, action potential amplitude; APD, action potential duration at different levels of repolarization (90% and 50%)