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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Every road leads to Rome: therapeutic effect and mechanism of the extracellular vesicles of human embryonic stem cell-derived immune and matrix regulatory cells administered to mouse models of pulmonary fibrosis through different routes

Fig. 8

IMRC-EVs inhibit TGF-β-induced EMT in vitro. Immunofluorescence staining of E-cad (a) and Col-I (b) expression in A549 cells with or without the treatment with 2 ng/mL TGF-β and IMRC-EVs for 48 h. Scale bar, 20 μm. c Western blot for E-cad, N-Cad and Fn protein expression in A549 cells, with or without 2 ng/mL TGF-β1 and IMRC-EVs treatment for 48 h. GAPDH was used as a loading control. d–f Quantification of the relative protein expression levels in lung tissues from mice relative to GAPDH. g, h qPCR for the relative expression of E-cad and Col-I mRNA in A549 cells, with or without 2 ng/mL TGF-β1 and IMRC-EVs treatment for 48 h. I The expressions of Smad2/3, active p- Smad2/3 factors, α-SMA and collagen-I were determined by Western blotting. The relative phosphorylation ratio is determined by p-Smad2/3 vs. total Smad2/3 and was presented graphically (j). Quantification of the relative Collagen I protein expression levels (k) and α-SMA protein expression levels (l)

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Stem Cell Research & Therapy

ISSN: 1757-6512