Fig. 5
a C57Bl6 mouse hearts were mounted on a Langendorff system. The ex vivo protocol comprises a 15 min-period of stabilization, followed by 30 min of global ischemia achieved by stopping the flow through the aorta (no-flow). Reperfusion was achieved by restoring the Tyrode perfusion during 60 min (IR group). In the MSC group, reperfusion was achieved with a solution of MSC cells prepared in a Tyrode buffer (2500 cells/mL) pre-treated or not by GW0742 at 1 µM. At the end of the protocol, hearts were collected and proteins extracted for further analysis. b, c, d Western blot analysis was performed from LV protein extracts from IR non-treated or MSC-treated murine IR hearts (ex vivo) with or without a pharmacological pre-treatment. Scatter dot blots and mean ± SD were plotted for Cleaved-caspase 3 (p** = 0.064 for IR, n = 10 vs MSC/GW0742, n = 10), for pAKT/AKT (p* = 0.0385 for IR, n = 8 vs MSC/GW0742, n = 8) and for pERK/ERK (pns = 0.2476 for IR, n = 6 vs MSC/GW0742, n = 6). Representative gel blots are presented for each protein for the three conditions (IR, MSC and MSC + GW0742). Vinculin or α-actinin were used as protein loading control. Data were compared using non parametric Kruskal–Wallis test (Dunn’s post hoc test)