Fig. 5

Limited LATS1 potentiates trophoblast endoreduplication. A Quantitative real-time PCR analysis of LATS1 overexpression in trophoblast cells. Differentiation was induced 6 h post-transfection and the cells were harvested after 72 h. B Quantitative real-time PCR analysis of Prl2c2 in LATS1 overexpressing cells under similar conditions described in A. C Confocal photomicrographic images of LATS1 overexpressing cells 72 h post-differentiation induction. The nuclei have been stained using Hoechst (blue) and counterstaining of the cytosolic actin filaments has been done using DyLight™ 554 Phalloidin (red). Boxed areas represent disintegration of the actin filaments which has been magnified in the rightmost panel. Scale bar: 10 µm. Magnification: × 88 D Quantitative assessment of the nuclear surface area/cell of trophoblast cells upon LATS1 overexpression using ImageJ software. E FACS profile showing the DNA content histogram (top) of the gated population in cells transiently transfected with LATS1 or empty vector control and stained with Hoechst. The percentage of cells with DNA content > 4 N is shown in the right panel. E The percentage of polyploid nuclei has been plotted from an average of six independent biological flow cytometric replicates. Values are represented as mean ± SEM from three independent biological replicates. Statistical analysis was performed using Student’s unpaired t-test, *p < 0.05; **p < 0.005; ***p < 0.0005