Fig. 3From: Treating intrauterine adhesion using conditionally reprogrammed physiological endometrial epithelial cellsMEECs express endogenous ER and PR and respond to estrogen stimulus. A mRNA expression of ERα and PR in MEECs. B Protein expression of ERα and PR in MEECs. MEECs at different passages were harvested, and the endogenous expression of ERα and PR was analyzed by RT-PCR and western blotting. β-actin is a loading control. The experiment was repeated three times. The representative data were shown. C MEECs respond to exogenous estrogen stimulus. Cells were treated with 17β-estradiol at different concentrations of 1 nM, 10 nM, 100 nM for 24 h. And then cells were harvested, and the expression of ERα and PR was analyzed by western blotting. β-actin is a loading control. The experiment was repeated three times. The representative data were shownBack to article page