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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Treating intrauterine adhesion using conditionally reprogrammed physiological endometrial epithelial cells

Fig. 3

MEECs express endogenous ER and PR and respond to estrogen stimulus. A mRNA expression of ERα and PR in MEECs. B Protein expression of ERα and PR in MEECs. MEECs at different passages were harvested, and the endogenous expression of ERα and PR was analyzed by RT-PCR and western blotting. β-actin is a loading control. The experiment was repeated three times. The representative data were shown. C MEECs respond to exogenous estrogen stimulus. Cells were treated with 17β-estradiol at different concentrations of 1 nM, 10 nM, 100 nM for 24 h. And then cells were harvested, and the expression of ERα and PR was analyzed by western blotting. β-actin is a loading control. The experiment was repeated three times. The representative data were shown

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