Fig. 6

Hypo-EVs rescued mitochondrial homeostasis in vivo. a Representative TEM images of kidneys on D 14 after I/R injury. Scale bar represents 500 nm. b, c Western blot was used to detect the protein expression of mitochondrial OXPHOS proteins (ATPB、SDHB and COX IV). The relative expression of ATPB, SDHB and COX IV were evaluated (n = 5–6). d Mitochondrial membrane potential was determined through a JC-1 probe in HK-2 cells after incubated with TGF-β1. Scale bar represents 100 μm. e Mito-SOX Red was used to observe the levels of intracellular ROS in the HK-2 cells after incubated with TGF-β1. Scale bar represents 100 μm. Data are expressed as mean ± SEM. *P < 0.05,** < 0.01