Fig. 1

Increased eNOS expression in high-grade prostate cancer and metastatic CRPC. a Comparison of mRNA expressions of three NOS isoforms showed that eNOS exhibited a consistent elevated expression pattern in metastatic CRPC tissues as compared to that in benign hyperplastic prostates and localized prostate cancer tissues, as revealed by two microarray expression datasets (GSE32269, GSE35988). b eNOS displayed statistical higher mRNA expression in high Gleason score prostate cancers, as revealed by a microarray expression dataset GSE21032. c Kaplan–Meier survival curves of cohort from TCGA using the GEPIA2 analysis tool revealed that prostate cancer patients with high eNOS expression level (top 20%) would have a significant shorter disease-free overall survival than patients with low eNOS expression level (bottom 20%). *P < 0.05, **P < 0.01, ***P < 0.001