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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Assessing the impact of gestational age of donors on the efficacy of amniotic epithelial cell-derived extracellular vesicles in experimental bronchopulmonary dysplasia

Fig. 1

Flow chart of experimental induced BPD bronchopulmonary dysplasia (BPD) model. C57/BL6 mice were time-mated and on day 16 of pregnancy (E16) injected with either 0.1 μg lipopolysaccharide (LPS) in 5 μL saline or an equal volume of vehicle into each amniotic sac. Pups were then allowed to deliver naturally at term. The pups were placed either in normoxia or in hyperoxia chambers on PND3.5 (12 h before hAEC treatment). On PND4, either hAECs (human amnion epithelial cells, 100,000 cells) or EVs (term/preterm extracellular vesicles, 10 μg) or saline was injected intravenously through the superficial temporal vein using the automated microinjection system as per the intra-amniotic injections. One cohort of mouse pups were culled on either PND7 or PND14. Another cohort of mouse pups were kept in hyperoxia chamber until PND28 when they were weaned and transferred to standard housing facilities. Animals were culled at 6 weeks of age

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Stem Cell Research & Therapy

ISSN: 1757-6512