Fig. 2

Human iPSC-derived NCSCs form spheroids with an enhanced regenerative secretome. A Size dependency of NCSC spheroid survival, Scale bar = 100 μm unless otherwise specified. B 3D quarter-cutaway of a spheroid stained for NCSC marker p75. Scale bar = 20 μm. C Regenerative secretome of NCSCs in various modalities. GF expression levels were reported relative to the GF with the lowest detected concentration, i.e., IGFBP-2 concentration in adherent 2D culture