Fig. 3

NCSC spheroids improve in vivo survival following transplantation. A, B NCSCs were subject to live–dead stain following ejection through the needle prior to transplantation. Green = live, Red = dead. A Single-cell suspension and B spheroids are shown. Scale bar = 100 µm. C Bioluminescent noninvasive tracking of luciferase-labeled NCSC survival (total flux, in p/s) after transplantation as single-cell suspension versus spheroids, n = 3 each. D Plot of total flux relative to original baseline directly following transplantation