Fig. 6

Pluripotency affects the expression of Mb. A Representative blotting of DP-MSCs protein extract at different times following osteogenic differentiation; bands immune-reacting for Runt-related transcription factor (RUNX2) and myoglobin (Mb) are shown; the graph on the right shows the normalized densitometric analysis of RUNX2 and of the 17 and 35 kDa Mb with values means ± S.E.M. of three independent experiments; *, P < 0.05, ***, P < 0.001 vs undifferentiated DP-MSCs. B Quantitative RT-PCR analysis of the transcript variants Mb-v2 and Mb-v13 in fibroblasts and fibroblast-derived iPSCs normalized to their expression in rhabdomyosarcoma cells (RD); means ± SEM of two independent measurements (biological replicates) each carried out in three technical replicates; **, P < 0.01 and ^##, P < 0.01 and ^###, P < 0.001 vs RD; the histogram on the right shows the relative amounts of Mb-v13 compared to that of Mb-v2 expressed as ratios of the relative 2^−ΔCt values; **, P < 0.01 and ^###, P < 0.001 versus RD