Fig. 1
From: Large-scale engineering of hiPSC-derived nephron sheets and cryopreservation of their progenitors
Generating hiPSC-derived nephron sheets using a template. A Schematic of the protocol for generating hiPSC-derived kidney organoids. hiPSCs are cultured on multiwell plates as a monolayer until day 7. Cells are dissociated and pipetted as clumps on transwell membranes and cultured until day 7 + 18 (4 mm diameter and 0.13 cm2 surface area). Scalebar inset: 5 mm. B, C Schematic of the protocol for generating hiPSC-derived nephron sheets. hiPSCs are differentiated in T75-culture flasks as a monolayer, followed by dissociation to single cells on day 7. Differentiated cells are either pipetted inside a ring (C, top) or pipetted cells are overlaid with a cover (C, bottom). Nephron sheets are cultured until day 7 + 18 and reach 18 mm diameter with 2.5 cm2 surface area. Scalebar inset: 5 mm. D Images of whole tissue sheet (left) and brightfield (middle), and immunofluorescence (right) of nephron sheets generated with a ring (top) or cover (bottom)