Fig. 10

Cell treatment upregulated growth factors in both types of skeletal muscle in 36-week-old SAMP10 mice. A, B mRNA expression levels of hepatic growth factor (HGF) in gastrocnemius muscle (A) and soleus muscle (B) were measured by quantitative RT-PCR (Cont, n = 7; Cell, n = 8). C Heat map representing the gene expressions of paired box-7 (PAX-7), myogenic differentiation antigen (MyoD), and HGF among all cell passages (P) (P2, P4, P6, P8). Analyzed clusters, identified based on hierarchical gene clustering, are highlighted in colored boxes: red box for PAX-7 overexpressed in P4, P6, and P8; yellow box for MyoD overexpressed in P4; blue box for HGF overexpressed in P4. D–F Quantitative RT-PCR of the mRNA expression levels of HGF, PAX-7, and MyoD among all experimental cell passages (n = 9 for each group). Data are mean ± SEM