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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: A reduced level of the long non-coding RNA SNHG8 activates the NF-kappaB pathway by releasing functional HIF-1alpha in a hypoxic inflammatory microenvironment

Fig. 3

SNHG8 inhibits NF-κB signaling pathway in PDLCs. Cells were transfected with the small interfering RNAs against SNHG8 (si-SNHG8), the siRNA control (si-NC) and recombinant fluorescently labeled lentivirus containing the full-length SNHG8 (SNHG8), together with the control (NC) to knockdown or overexpress its expression. A Fluorescence microscopy show the transfection efficiency in PDLCs of NC and SNHG8 groups. Scale bar, 100 μm. B qRT-PCR results show the relative SNHG8 expression in the SNHG8 knockdown and overexpression groups. C qRT-PCR results show the mRNA expression of IL-1β, IL-6, and IL-8 after TNF-α stimulation. D qRT-PCR results show the relative RNA expression of IL-1β, IL-6, and IL-8 following knockdown or overexpression of SNHG8 under TNF-α stimulation. E Western blot analyses show the effects of SNHG8 knockdown or overexpression on the activation of NF-κB pathways induced by TNF-α in PDLCs. Histograms show the quantification of band intensities. (Analysis of variance *p < 0.05; **p < 0.01; ***p < 0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512