Fig. 3

Hairpoor mouse displays the loss of label-retaining HFSC and increased cell proliferation in HF. a Immunofluorescence staining of BrdU-LRC in the CD34⁺ bulge at P70. White arrowhead indicates LRC in bulge. Bulge, Bu; scale bar = 25 μm. b Average number of LRC in bulge at P70. Data are mean ± SD. *P < 0.05. Mouse number n = 3 per genotype. Observed follicle number per each mouse n = 10. c Immunofluorescence staining of BrdU (1 h chase) at anagen II HFs (the wild-type mouse at P23 and the hairpoor mouse at P21). Scale bar = 100 μm. a,c Nuclei were counterstained with DAPI. d Quantification of BrdU positive cells in follicle. Data are mean ± SD. **P < 0.01, ***P < 0.001. n = 3 per genotype. Number of HFs counted in each mouse ≥ 10