Fig. 1

Using the Z-stack imaging technique could achieve high-quality images of 3D organoids. A Representative pictures in the same field show that it is difficult for researchers to catch all organoids in a single-layer image. B Schematic diagram of Z-stack imaging technique. C, D Images obtained by continuous screening of the same well from multiple layers were used to make a final image (C bright field, D fluorescent field). Top: Representative single-layer images. Bottom: Representative images after executing Z-stack techniques on multiple layers. *Organoids that were blurred in the single-layer photograph were clear in the Z-stacked photograph