Fig. 5
From: Assay establishment and validation of a high-throughput organoid-based drug screening platform
Optimization of Calcein-AM fluorescent staining method. A, B Variation of tumor organoid labeling rates with different staining times and dye concentrations. C, D 0.1 mM Cu2+ effectively reduced the staining background during the staining process of mouse small intestinal organoids. Three wells were measured for each group and data of 27 points were obtained. ****P < 0.0001