Fig. 7
From: Assay establishment and validation of a high-throughput organoid-based drug screening platform
Validation of a high-throughput organoid imaging assay by testing the radiation-mitigating effect on intestinal organoids. A Workflow for verifying the radiation-mitigating effects of drugs on mouse intestinal organoids after 8 Gy irradiation. B The radiation-mitigating effects of bFGF, NAM, SGC on small intestinal organoids at d5 after 8 Gy irradiation. C, D Compared with the vehicle group, the bFGF (200 ng/mL), NAM (10 mM), and SGC (5 μM) groups had significantly higher survival rates of small intestinal organoids, and the total organoids sizes per well were larger. 57, 82, 145, and 241 surviving organoids from five images were analyzed in the Vehicle, bFGF, NAM, and SGC group, respectively. Data are presented as the mean \(\pm\) SD (n = 5). *P < 0.05, ***P < 0.001, ****P < 0.0001