Fig. 1

Morphology, differentiation potential and immunophenotyping profiles of CL-, CPJ- and FP-MSCs derived from same donors. a Schematic of the isolation of cells from the cord lining of human umbilical cord (CL), cord–placenta junction (CPJ) and fetal placenta (FP). b Time required for the cell density of perinatal tissue culture reached 70% (P0). c Cell growth curve (P3). d Fibroblast-like morphology of MSCs at passage 3. e Surface marker expression of three types of MSCs analyzed by flow cytometry (CD73). f Quantitative profile of surface markers expression. g Adipogenic differentiation (Oil Red O staining); osteogenic differentiation (Alizarin Red staining); and chondrogenic differentiation (Alcian Blue staining). * represents a significant difference (p < 0.05). ** represents a significant difference (p < 0.01). *** represents a significant difference (p < 0.001), n = 3 for each type of MSCs