Fig. 1

Cdc42 regulates activin B-mediated ADSCs migration in vitro. A Pull-down assays were performed to detect the amounts of GTP-bound Cdc42 in ADSCs, ADSCs treated with activin B, respectively. B The ratios of GTP-Cdc42 versus total Cdc42 levels were analyzed. C Scratch wound healing assays were performed to detect the migration of cells treated by seven methods, and photographs were taken at 0, 24, and 48 h after scratch injury. Scale bar = 500 μm. D The healing rates were quantified by measuring the area of the injured region. E Transwell assays were performed to detect the invasion of these cells, and photographs were taken at 24 h after seeds. Scale bar = 200 μm. F The migrated cells were counted. All values are expressed as mean ± SD from three independent repeats. *P < 0.05