Fig. 3
From: High-efficient serum-free differentiation of endothelial cells from human iPS cells
Characterization of hiPSC-ECs. A, C and E Representative plots, and B, D, and F biological replicates (n = 6) of FC analysis on day 6 of hiPSC-ECs differentiation for CD31-APC:VE-Cadherin-PE, CD31-APC:CD34-FITC and CD31-APC:CD184-PE double positive cells. Data were expressed mean ± S.D. P < 0.05 was considered as a significant difference versus control group. G hiPSC-ECs forming vascular tube-like structures on thick Matrigel layers. Images were obtained 5 h after seeding. Scale bar = 100 µm at 10X and 20X microscope lens power magnifications. H LDL uptake assessment of hiPSC-ECs. I Immunostaining with antibodies against LDL receptor (green). hiPSC-ECs were incubated with LDL-550 (red) for 5 h. J Staining with antibodies against nitric oxide synthase (anti-eNOS; red), and Hoechst against nuclei (blue). Scale bar = 100 μm