Fig. 2

Fbxw11 plays a negative role in the maintenance of HSPCs in vitro. A, B LSK cells expressing high levels of Fbxw11 variants were established by retroviral infection and verified by real-time RT-PCR (A) and Western Blotting (B). C, D Colony-forming capacity of LSK cells overexpressing Fbxw11 variants was analyzed by colony-forming assays. The numbers of total colonies as well as GEMM, BFU-E, GM, G and M colonies (C) and the percentages (D) are plotted, n = 3. E Percentage of GMPs was tested by FACS 48 h after LSK cells were infected with retroviruses. F LSK cells overexpressing Fbxw11 variants were stained with Ki-67 and Hoechst 33342. Typical FACS results and the columns of the percentage of G0 phase LSK cells (bottom left quadrant) are shown, n = 3. G–H The expression of stem cell homeostasis-associated genes (G) and proliferation-associated genes (H) in LSK cells overexpressing Fbxw11 variants was studied by quantitative real-time PCR, n = 3. Data are presented as mean ± SEM, three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001