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Fig. 10 | Stem Cell Research & Therapy

Fig. 10

From: Human umbilical cord-derived mesenchymal stem cells not only ameliorate blood glucose but also protect vascular endothelium from diabetic damage through a paracrine mechanism mediated by MAPK/ERK signaling

Fig. 10

Western blot of protein expressions related to the MAPK/ERK signaling pathway in HUVECs and thoracic aorta. A Representative images of ERK, p-ERK and GADPH in HUVECs; B Histogram of statistical analysis on ERK and p-ERK protein levels in HUVECs; C Representative images of ERK, p-ERK and GADPH in thoracic aorta; and D Histogram of statistical analysis on ERK and p-ERK protein levels in thoracic aorta. For statistical analysis of the protein expression, Image-J software (Version 1.49, National Institutes of Health, Bethesda, USA) was applied to quantify the gray value of each protein blot, and the ratio of gray value of the target blot (ERK and p-ERK) to that of the internal reference blot (GADPH) was calculated as the relative protein level in the histogram. All data were repeated for three times and added in supplementary data. Data was mean ± SD, *P < 0.05 and **P < 0.01 versus control level, #P < 0.05 and ##P < 0.01 versus model level

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Stem Cell Research & Therapy

ISSN: 1757-6512