Potency assays for human adipose-derived stem cells as a medicinal product toward wound healing

Table 2 In vitro biological potency assays testing the effects of hASCs on cells relevant for the inflammation phase of wound healing

MoA	Target	Assay	References
PBMC proliferation	DNA	3H-Thymidine	[25, 51]
	Intracellular lysine residues	CFSE	[26]
T cell proliferation	DNA	3H-Thymidine	[27]
	Intracellular lysine residues	CFSE	[26, 28]
M1 to M2 transformation of macrophages	ARG1	qPCR	[31, 36]
	CD206	FC	[29,30,31]
		qPCR	[28]
	IL-10	ELISA	[32, 33]
		FC	[34]
		qPCR	[28, 31, 32]
		WB	[31]
	IL-12	IF	[31]
		qPCR	[34]
		WB	[31]
	iNOS	qPCR	[28, 31, 36]
	TGF-β	ELISA	[32, 33]
		qPCR	[28]
	TNFα	qPCR	[28, 29, 32, 34]
		ELISA	[29, 32, 33]

MoA, mode of action; PBMC, peripheral blood mononuclear cell; DNA, deoxyribonucleic acid; CFSE, carboxyfluorescein succinimidyl ester; ARG1, arginase 1; qPCR, quantitative polymerase chain reaction; CD, cluster of differentiation; FC, flow cytometry; IL10/12, interleukin 10/12; ELISA, enzyme-linked immunosorbent assay; WB, western blotting; iNOS, inducible nitric oxide synthase; TGF-β, transforming growth factor-beta; TNFα, tumor necrosis factor-alpha

ISSN: 1757-6512