Potency assays for human adipose-derived stem cells as a medicinal product toward wound healing

Table 4 In vitro biological potency assays testing the effects of ASCs in the remodeling phase of wound healing

MoA	Target	Assay	References
Collagen deposition	Collagen I	qPCR	[38, 41, 52, 56, 57, 62,63,64]
		IF	[57]
		WB	[55, 57, 62, 64]
	Collagen III	qPCR	[38, 41, 52, 56, 57, 62, 64]
		IF	[57]
		WB	[57, 64]
ECM modulation	MMP-1	qPCR	[57, 62, 65]
		WB	[55]
Scar contraction	α-SMA	qPCR	[56, 64]
		WB	[41, 64]

MoA, mode of action; qPCR, quantitative polymerase chain reaction; IF, immunofluorescence microscopy; WB, western blotting; MMP-1, matrix metalloproteinase-1; α-SMA, Smooth muscle α-actin

ISSN: 1757-6512