IL10-MSCs inhibiting MAPK JNK signaling in HFD-induced obese mice and reducing adipocyte differentiation in 3T3-L1 adipocytes. A The phosphorylations of MAPK p38, ERK1/2, and JNK in visceral white adipose tissues were detected by western blot analysis. B-D The quantified data of MAPK p38, ERK1/2, and JNK phosphorylation were analyzed by Image J, respectively. The phosphorylation of JNK was significantly activated in HFD-induced obese mice. MSCs, especially IL10-MSCs administration decreased the JNK phosphorylation level. E–F Blocking of JNK signaling by SP600125 inhibited the adipocyte differentiation in 3T3-L1 adipocytes. G-H Condition medium from MSCs and IL10-MSCs effectively inhibited the adipocyte differentiation in 3T3-L1 adipocytes (N = 3 per group) (*p < 0.05; **p < 0.01; ***p < 0.001).