Fig. 6

Effect of OE-glud1 BMSCs on the ENS regeneration in the BAC mice. A Representative immunofluorescence images in gastric myenteric plexus of GFAP (red) in each group. B Representative immunofluorescence images in gastric myenteric plexus of HuC/D (green) in each group. C Representative immunofluorescence images of GFAP (red) and HuC/D (green) in each group, the nuclei (blue). D Representative immunofluorescence images in gastric myenteric plexus of S100B (red) in each group. E Representative immunofluorescence images in gastric myenteric plexus of β-Tubulin (green) in each group. F Representative immunofluorescence images of S100B (red) and β-Tubulin (green) in each group, the nuclei (blue). G The protein expression of GFAP (Con:1, BAC: 0.17 ± 0.08, BAC + BMSCs-NC: 0.43 ± 0.10, BAC + BMSCs-OE: 0.88 ± 0.12)/HuC/D (Con: 1, BAC: 0.20 ± 0.08, BAC + BMSCs-NC: 0.54 ± 0.08, BAC + BMSCs-OE: 0.76 ± 0.10)/S100B (Con: 1, BAC: 0.21 ± 0.12, BAC + BMSCs-NC: 0.41 ± 0.10, BAC + BMSCs-OE: 0.77 ± 0.10)/β-Tubulin (Con: 1, BAC: 0.05 ± 0.02, BAC + BMSCs-NC: 0.37 ± 0.09, BAC + BMSCs-OE: 0.70 ± 0.07) in the gastric tissues was examined by immunoblotting. H The statistics results of GFAP/HuC/D/S100B/β-Tubulin protein expression in the gastric tissues. Control: the control C57 mice; BAC: benzalkonium chloride-treated mice; BAC + BMSCs (NC-Glud1): BAC mice transplanted with BMSCs-NC; BAC + BMSCs (OE-Glud1): BAC mice transplanted with BMSCs-OE. Results were expressed as mean ± SD (n = 4 per group). *P < 0.05, **P < 0.01, NS: no significance