Fig. 1
ASCs cultured in microwells at different seeding densities formed spheroids of different sizes in 7 days. a Representative microscopic images of ASC spheroids formed by respective cell number (2K, 4K, and 8K; K: 1 × 103 cells; scale bar = 200 µm). b Ferret diameter of 2K, 4K and 8K spheroids was estimated at day 1, 4 and 7 (n = 26; ***p < 0.005 relative to the 2K spheroid group). c Representative H&E histological images and scanning electron microscopic (SEM) images of 2K, 4K and 8K ASC spheroids (scale bar = 50 µm). d Second harmonic generation images of ASC sheet after 7 days of culturing (scale bar = 50 µm). e Representative SEM images of ASC sheets in top view and cross section (scale bar = 20 µm)