Fig. 2

Analysis of RNA sequencing data in ASC spheroids and sheets relative to monolayer ASCs. a Venn diagram of the number of differential expression genes (> 2x) in spheroid and sheet groups compared to the monolayer group. b Functional analysis of pathways with significant differential expression genes in spheroid and sheet groups. Two angiogenesis-related pathways were found in the spheroid group (red square), but none was identified in the sheet group. c Angiogenesis-associated genes with differential expression between the spheroid and monolayer groups (> 2x) were selected to generate a heatmap using the log2 fold change values relative to the monolayer group