Fig. 5
![Fig. 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13287-022-02948-3/MediaObjects/13287_2022_2948_Fig5_HTML.png)
Expression of endothelial markers and angiogenic growth factors in ASC spheroid sheets. a CD31 immunofluorescence of the PKH26-labeled ASC spheroids seeded on ASC sheets. b Representative H&E histological image of the cross section of a ASC spheroid sheet (arrow indicates a seeded ASC spheroid; scale bar = 100 µm). c Gene expression analysis of HGF, VEGF and FGF2 in monolayer ASC, ASC sheet and ASC spheroid sheet. The gene expression was normalized to monolayer ASC. d Release of HGF, VEGF and FGF2 from monolayer ASC, ASC sheet and ASC spheroid sheet into the medium as determined by ELISA. (n = 3; *p < 0.05, **p < 0.01, ***p < 0.005 from the monolayer ASC group; #p < 0.05, ###p < 0.005 between the indicated groups)