Fig. 5

Expression of endothelial markers and angiogenic growth factors in ASC spheroid sheets. a CD31 immunofluorescence of the PKH26-labeled ASC spheroids seeded on ASC sheets. b Representative H&E histological image of the cross section of a ASC spheroid sheet (arrow indicates a seeded ASC spheroid; scale bar = 100 µm). c Gene expression analysis of HGF, VEGF and FGF2 in monolayer ASC, ASC sheet and ASC spheroid sheet. The gene expression was normalized to monolayer ASC. d Release of HGF, VEGF and FGF2 from monolayer ASC, ASC sheet and ASC spheroid sheet into the medium as determined by ELISA. (n = 3; *p < 0.05, **p < 0.01, ***p < 0.005 from the monolayer ASC group; ^#p < 0.05, ^###p < 0.005 between the indicated groups)