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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Enhanced angiogenic potential of adipose-derived stem cell sheets by integration with cell spheroids of the same source

Fig. 5

Expression of endothelial markers and angiogenic growth factors in ASC spheroid sheets. a CD31 immunofluorescence of the PKH26-labeled ASC spheroids seeded on ASC sheets. b Representative H&E histological image of the cross section of a ASC spheroid sheet (arrow indicates a seeded ASC spheroid; scale bar = 100 µm). c Gene expression analysis of HGF, VEGF and FGF2 in monolayer ASC, ASC sheet and ASC spheroid sheet. The gene expression was normalized to monolayer ASC. d Release of HGF, VEGF and FGF2 from monolayer ASC, ASC sheet and ASC spheroid sheet into the medium as determined by ELISA. (n = 3; *p < 0.05, **p < 0.01, ***p < 0.005 from the monolayer ASC group; #p < 0.05, ###p < 0.005 between the indicated groups)

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