Fig. 1

Silencing of OSMR in BMSCs enhanced osteogenesis and inhibited adipogenesis. Silencing of OSMR in BMSCs after the infection of control or Osmr-KD lentivirus was detected using qRT-PCR (A) and Western blotting (B). The mRNA level of Lifr was detected using qRT-PCR (C). The effect of OSMR silencing on the viability of BMSCs was detected by CCK-8 assay (D). ALP staining (E) and alizarin red staining (F) of differentiated osteoblasts were done. The mRNA (G) and protein (H) levels of osteogenic factors were examined. Lipid droplet formation in differentiated adipocytes was detected by staining with oil-red O (I). Oil-red O stain was extracted and absorbance was measured by spectrophotometry at 520 nm (J). The mRNA (K) and protein (L) levels of adipogenic factors were examined. Scale bar in (I): 50 μm. Values are mean ± SD, n = 3 in (A–C, G, H, J–L), n = 9 in (D). *p < 0.05 vs. Control LV