Fig. 3

OSMR inactivated ERK signaling pathway and suppressed autophagy. Levels of SHC1 and ERK1/2 in ST2 were examined 48 h after OSMR overexpression (A) or silencing (B) by Western blotting. The mRNA (C: overexpression; E: silencing) and protein (D: overexpression; F: silencing) levels of the key factors for autophagy were examined 48 h after transfection. The level of LC3 puncta formation was analyzed in ST2 cells infected with GFP-LC3 adenovirus for 24 h followed by transfection with Osmr siRNA or control siRNA for 24 h. Representative confocal microscopic pictures are shown (G). Scale bar: 10 μm. Values are mean ± SD (n = 3). *p < 0.05 vs. Vector or Ctrl siRNA