Fig. 6

Simulated p-KAP1 (S824) improved locomotor activity of rats subjected to cerebral I/R. A Experimental design was illustrated schematically. A total of 50 rats were stochastic fall into 5 groups: the (1) NC (Scramble AAV 2/9), (2) NC + I/R (Scramble AAV 2/9 + I/R), (3) WT + I/R (AAV2/9-KAP1 + I/R), (4) S824D(AAV2/9-KAP1-S824D + I/R), (5) S824A(AAV2/9-KAP1-S824A + I/R) groups. Adeno-associated virus-mediated KAP1 and its mutation overexpression were administered 14 days before. After ischemia for 1.5 h, the rats were under reperfusion by pulling out the plug. B–E Effects of KAP1 and its mutations on locomotor activity of rats subjected to I/R. The total distance, speed, and time in the center were investigated by an open field test. Data were expressed as mean ± SEM (n = 3–6), ^*P < 0.05, ^**P < 0.01 versus NC + I/R group; ^&P < 0.05 versus WT + I/R group. F A balance beam test was performed on the sixth day after cerebral ischemia. Data were expressed as mean ± SEM (n = 3–4), ^***P < 0.001 versus NC + I/R group