Fig. 1
![Fig. 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13287-022-02963-4/MediaObjects/13287_2022_2963_Fig1_HTML.png)
ADSC-sEVs were internalized by neurons to inhibit apoptosis in vitro. a Representative images of neurons incubated with Dil-labeled sEVs. The nuclei of neuron were stained with DAPI (blue). b TUNEL staining (red) showing apoptotic rate of primary neurons. Cell nuclei were counterstained with DAPI (blue). c Annexin V-FITC/PI double staining was determined by flow cytometry. d Quantitative estimation of the apoptotic rate in each group. ADSC-sEVs significantly reduced OGD/R-induced apoptosis. e Quantitative estimation of apoptosis rate of neurons with flow cytometry. f Expression of apoptosis-related proteins as measured by Western blotting analysis. g Relative expression levels of apoptosis-related proteins normalized to GAPDH. *p < 0.05 compared with the control group, #p < 0.05 compared with the OGD/R group