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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Endoplasmic reticulum stress modulates the fate of lung resident mesenchymal stem cell to myofibroblast via C/EBP homologous protein during pulmonary fibrosis

Fig. 4

CHOP overexpression facilitates the TGFβ1-induced myofibroblast differentiation of LR-MSC. A Isolated LR-MSC was cultured in 6-well plates and incubated with 5 ng/mL TGFβ1 for 48 h. The shape of TGFβ1-treated LR-MSC changed and showed characters of myofibroblasts with thin and elongated appearance under phase-contrast microscopy. B Western blots show the effects of CHOP overexpression by Lenti-CHOP on TGFβ1-induced myofibroblast differentiation. C Western blots show the effects of CHOP knockdown by Lenti-shCHOP on TGFβ1-induced myofibroblast differentiation. D Immunofluorescence of LR-MSC for the expression of Collagen I and α-SMA under Lenti-CHOP or Lenti-shCHOP conditions. Arrows indicate the extracellular collagen fibers

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Stem Cell Research & Therapy

ISSN: 1757-6512