Fig. 4

Characterization of hematopoietic specification after the hematopoietic differentiation from hESCs. A CLSM images of the immunostainings for VE-Cadherin and RUNX1 in adherent cells at day 15 during the hematopoietic specification after the differentiation. The colors of green, red and blue represent the staining for RUNX1, VE-Cadherin and nuclei. (Original magnification, 20×, scale bar 40 μm, upper panel; 40×, scale bar 50 μm, lower panel). B CLSM images of the immunostainings for CD45 and CD34 in suspension cells at day 15 during the hematopoietic differentiation. The colors of green, red and blue represent the staining for CD34, CD45 and nuclei. (Original magnification, 20×, scale bar 40 μm, upper panel; 40×, scale bar 50 μm, lower panel). C Analysis of hematopoietic gene expressions at defined stages during the differentiation by RT-qPCR (n = 3, mean ± SEM). D Flow analysis for HPCs (CD34⁺CD45⁺ cells) during the hematopoietic development after the differentiation. Data represent the mean ± SEM. *p  < 0.05, **p < 0.01, and ns (no significance)