Fig. 3

AREG enhances the oriented differentiation potential of DPSCs. A Alizarin Red S staining of DPSCs after 14 days’ culture with a range of AREG concentrations in mineralizing medium. B Quantitative analysis of Alizarin Red S staining (n = 5, **P < 0.05). C Western blot analysis showing expression of the odontoblastic markers DSPP, RUNX2, BSP, and OCN. The full-length gels and blots are included in Additional file 1: Fig. S1A. D ImageJ software analysis of the gray level of the panel. Data are expressed as means ± SDs, n = 3, *P < 0.05, **P < 0.01