Fig. 4

Defects in the differentiation of naïve ESCs by Shp2 depletion. A Light microscopic images of embryonic body (EB) cultured for 3 days of WT or KD ESCs (scale bars, 500 μm) (left), Graphical presentation of relative mRNA expression of typical naïve pluripotency and core pluripotency markers (right) of WT (blue) and KD (red). B Light microscopic images of WT and KD before [LIF/2i] or 48 h after differentiation [(-)LIF/2i].C Relative mRNA expressions of naïve pluripotency (top) and core pluripotency (bottom) markers at indicative time during spontaneous differentiation of WT (blue) and KD (red). D Representative images of teratomas formed in mouse testes at 6 weeks after injection of WT (top) and KD (bottom) ESCs. E Graphical presentation of teratoma volume [V = a × b × c × π/6 (a = length, b = width, and c = depth] of total 13 teratoma from WT or KD ESCs (left), formed three different areas (right), volumes of normal testes after teratoma injection were considered as 0. F Hematoxylin & eosin (H&E) (for ectoderm and endoderm) and Masson’s trichrome (for mesoderm) staining of teratoma section from WT and KD, black arrows represent typical tissues for ectoderm, mesoderm and endoderm. Uncharacterized tissue from teratoma of KD were shown in the box