Fig. 5

Histochemical analysis of collagen deposition in wounds treated by PF-127/hADSCs-Exos. a Masson staining of collagens deposition in different groups on days 4, 7, 10, and 13 post-wounding. Scale bar = 50 µm. b Quantification of collagens deposition in different groups. c Immunohistochemistry staining images for collagen I at 7, 13 days post-wounding. Scale bar = 20 µm. d Relative density analysis of collagen I. e Immunohistochemistry staining images for collagen III at 7, 13 days post-wounding. Scale bar = 20 µm. f Relative density analysis of collagen III. g Relative density analysis of collagen I/collagen III ratios at 7 and 13 days after treatment. In b, d, f, and g, data are shown as mean ± SEM; n = 6 for each group. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001, ****p-value < 0.0001 vs vehicle control group