Fig. 5

CCL20 promotes ESC proliferation and migration via the CCR6/TFEB/Autophagy pathway. A Proliferative capability of ESCs, incubated with rCCL20 in response to TFEB-OE with and without 3-MA (inhibitor of autophagy), assessed by CCK8 assay. B Proliferative capability of ESCs in different groups assessed by Edu assay. (original magnification 200 ×) C Quantification of Edu incorporation rate. D Migration capability of ESCs in different groups assessed by wound healing assay. (original magnification 40 ×) E Quantification of wound closure rate. F Migration capability of ESCs in different groups assessed by Transwell assay. (original magnification 200 ×) G Quantification of migration cells per field at 200 × magnification. H ELISA analysis of CCL20 in macrophages culture supernatant with or without co-cultured with ESCs. I The mRNA level of CCL20 in macrophages with or without co-cultured with ESCs by qRT-PCR. J The mRNA level of IL-6 in ESCs with or without co-cultured with macrophages by qRT-PCR. K The mRNA level of TNF-α in ESCs with or without co-cultured with macrophages by qRT-PCR. Data are presented as the mean ± SD of n = 3 independent experiments. Statistical analysis was performed using one-way ANOVA with Tukey post hoc (A, C, E, G), Mann–Whitney test (I, J, K) or Student’s t test (H). ***p < 0.001, **p < 0.01, *p < 0.05, ns, non-significant