Fig. 4

MSC-sEV exerted repairing effects through PTEN/AKT signaling pathway. A Western blot analysis for the expressions of cell signaling pathway in RPE cells after treatment. B Immunofluorescence staining of PTEN and p-AKT in RPE cells after treatment. Scale bars, 25 μm. C Representative immunohistochemistry staining images of PTEN and p-AKT in retinal tissues. Scale bars, 100 μm. D Western blot analysis for the expressions of PTEN and p-AKT in retinal tissues. E Western blot analysis for the expressions of PTEN, p-AKT and NRF2 in RPE cells transfected with PTEN siRNA. F Representative immunofluorescence staining images of NRF2 in RPE cells transfected with PTEN siRNA. Scale bars, 25 μm. G Detection of ROS generation in RPE cells transfected with PTEN siRNA. Scale bars, 100 μm. H CCK8 assay for the proliferation ability of RPE cells transfected with PTEN siRNA (n = 4). I Western blot analysis for the expressions of PCNA, Bcl-2 and Bax in RPE cells transfected with PTEN siRNA. All data are presented as means ± SEM. ns, not significant, ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001