Fig. 6

MiR-21 directly targeted BTG2 in H9c2 cells. A Predicted miR-21 target sequence in BTG2-3′ UTRs. Target sequences of BTG2-3′ UTRs were mutated. B Luciferase assay of 293T cells transfected with BTG2-3′ UTR-WT or BTG2-3′ UTR-Mut reporter together with mimics NC or miR-21-5p mimics (n = 3). C qRT-PCR analysis of BTG2 level in H9C2 cells treated with mimics NC and miR-21-5p mimics (n = 3). D Western blot analyzed BTG2 protein levels in H9c2 cells treated with control, mimics NC and miR-21-5p mimics. Relative protein levels were presented as the average expression normalized to β-Tubulin (n = 3). E qRT-PCR analysis of BTG2 level in H9c2 cells treated with IFN-γ-Exo and si-IFN-γ-Exo (n = 3). Data were presented as mean ± SEM. Statistical analysis was performed with one-way ANOVA followed by Bonferroni’s correction. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001