Table 2 Sequence of primers used for quantitative RT-PCR
From: Anti-fibrotic effect of a selective estrogen receptor modulator in systemic sclerosis
Target name | Direction | Primer sequence | Size |
|---|---|---|---|
hOCT4 | Forward | ACCCCTGGTGCCGTGAA | 190 |
| Â | Reverse | GGCTGAATACCTTCCCAAATA | Â |
hCD44 | Forward | AAGGTGGAGCAAACACAACC | 151 |
| Â | Reverse | AGCTTTTTCTTCTGCCCACA | Â |
hCOL1A1 | Forward | CCCCTGGAAAGAATGGAGATG | 148 |
| Â | Reverse | TCCAAACCACTGAAACCTCTG | Â |
hCOL1A2 | Forward | GGATGAGGAGACTGGCAACC | 77 |
| Â | Reverse | TGCCCTCAGCAACAAGTTCA | Â |
hCOL3A1 | Forward | CGCCCTCCTAATGGTCAAGG | 161 |
| Â | Reverse | TTCTGAGGACCAGTAGGGCA | Â |
hVimentin | Forward | GAGAACTTTGCCGTTGAAGC | 170 |
| Â | Reverse | TCCAGCAGCTTCCTGTAGGT | Â |
hPAX6 | Forward | GTCCATCTTTGCTTGGGAAA | 110 |
| Â | Reverse | TAGCCAGGTTGCGAAGAACT | Â |
hSOX1 | Forward | CACAACTCGGAGATCAGCAA | 133 |
| Â | Reverse | GGTACTTGTAATCCGGGTGC | Â |
hNp63 | Forward | GGAAAACAATGCCCAGACTC | 294 |
| Â | Reverse | GTGGAATACGTCCAGGTGGC | Â |
hKRT5 | Forward | ACCGTTCCTGGGTAACAGAGCCAC | 198 |
| Â | Reverse | GCGGGAGACAGACGGGGTGATG | Â |
hKRT14 | Forward | GCAGTCATCCAGAGATGTGACC | 181 |
| Â | Reverse | GGGATCTTCCAGTGGGATCT | Â |
hGAPDH | Forward | ACCCACTCCTCCACCTTTGA | 110 |
| Â | Reverse | CTGTTGCTGTAGCCAAATTCGT | Â |
mCOL1A1 | Forward | GCAACAGTCGCTTCACCTACA | 138 |
| Â | Reverse | CAATGTCCAAGGGAGCCACAT | Â |
mCOL3A1 | Forward | TGAGCGTGGCTATTCCTTCGT | 76 |
| Â | Reverse | GCCGTGGCCATCTCATTTTCAA | Â |
mACTA2 | Forward | GTTCTAGAGGATGGCTGTACTA | 108 |
| Â | Reverse | TTGCCTTGCGTGTTTGATATTC | Â |
mGAPDH | Forward | ACCCCAGCAAGGACACTGAGCAAG | 92 |
| Â | Reverse | TGGGGGTCTGGGATGGAAATTGTG | Â |