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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: A gene signature is critical for intrahepatic cholangiocarcinoma stem cell self-renewal and chemotherapeutic response

Fig. 7

4-key-genes promote ICC stemness features in a MAT2A-dependent manner. A Tumorspheres (TS) of primary ICC cells were treated with serum (adherent cell, Adh) or shRNA hairpin against SDHAF2, MRPS34, MRPL11 and COX8A (shSDHAF2-TS, shMRPS34-TS, shMRPL11-TS and shCOX8A-TS). Scale bar, 100 μm. B Methionine metabolomic comparison in different subgroups of primary ICC cells. C The correlation between SDHAF2 (or MRPS34) and key enzymes of methionine cycle in primary ICC cells. D Vector and Lv-SDHAF2 HCCC9810 cells (or Lv-MRPS34 HCCC9810 cells) were treated with or without FIDAS-5, and then the expression of pluripotent transcription factors was analyzed by qRT-PCR. (*p < 0.05, **p < 0.01, ***p < 0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512