Fig. 2

Notch-activated MSCs reduce macrophage/neutrophil infiltration and hepatocellular apoptosis after APAP challenge. A, B Immunohistochemistry staining and quantification of F4/80⁺ macrophages and Ly6G⁺ neutrophils in livers (n = 5 mice/group). Scale bars: 50 μm. C Quantitative RT-PCR-assisted detection of TNF-α, Il-1β, Mcp-1 and Cxcl-1 expression. D Representative images of TUNEL-staining liver sections and quantification of TUNEL-positive nuclei per high-power field (HPF) for at least 10 fields per sample (n = 5 mice/group). Scale bars, 50 μm. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01