Fig. 3
From: Macrophage-derived apoptotic vesicles regulate fate commitment of mesenchymal stem cells via miR155
Macrophage-derived apoVs inhibited osteogenesis of MSCs in vitro. A ApoVs inhibited osteogenic differentiation as indicated by ALP staining. B ApoVs inhibited osteogenic differentiation as indicated by ALP quantification. C ApoVs inhibited the mRNA expression of ALP and RUNX2 detected by RT-qPCR. D ApoVs inhibited osteogenesis as indicated by ARS staining. E ApoVs inhibited osteogenesis as indicated by ARS quantification. F ApoVs reduced the mRNA expression of RUNX2 and BGLAP detected by RT-qPCR. G Western blot showed that the protein expression of RUNX2 was down-regulated in the group treated with apoVs compared with the group without apoVs. H Western blot quantification showed the same result as Fig. 3G. **P < 0.01, ***P < 0.001