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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Extracellular vesicles derived from human umbilical cord mesenchymal stem cells alleviate osteoarthritis of the knee in mice model by interacting with METTL3 to reduce m6A of NLRP3 in macrophage

Fig. 7

HucMSCs-EVs facilitate the migration of chondrocyte and inhibit the degradation of ECM by downregulating METTL3 in macrophage. A. Migration of chondrocytes was observed using transwell assay. Scale bar = 200 μm. B. Images of cell scratch area healing assay on chondrocytes were obtained under a light field of microscope at 0 h, 24 h, and 48 h. C. The number of migratory cells was calculated and analyzed in each group (n = 3). Scale bar = 200 μm. D. The healing area of a scratch wound was analyzed in each group (n = 3). E. Western blot analysis for COL2A1, Aggrecan, ADAMTS5, and MMP13 in each group. F. Quantification of the density of immunoreactive bands normalized to tubulin in each group (n = 3). Data are presented as mean ± SD. ns, no significance; *p < 0.05; **p < 0.01; ***p < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512