Table 1 Oligonucleotide primers and PCR conditions in RT-qPCR
Genes | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
VEGF | GCTCTACCTCCACCATGCCA | AGCTCATCTCTCCTATGTGC |
Integrin αV | TGCATCAACCTTAGCTTCTGCCT | ACCAGCAGGCGGCTCTGGTTCAC |
Integrin α5 | GACAGGGAAGAGCGGGCACTATGG | GTCCCTTCCCGGCCGGTAAAACTC |
Integrin β1 | TGCCAGCCAAGTGACATAGAGA | ATCCGTTCCAAGACTTTTCACAT |
Integrin β3 | CTGCCGTGACGAGATTGAGT | TGCCCCGGTACGTGATATTG |
Integrin β5 | ACCTGGAACAACGGTGGAGA | AAAAGATGCCGTGTCCCCAA |
β-Actin | CTACAATGAGCTGCGTGTGGC | CAGGTCCAGACGCAGGATGGC |