Fig. 5

CEFFE inhibited apoptosis in CTX-damaged KGN cells. A KGN cell apoptosis was measured by Annexin V APC/7-AAD and flow cytometry. B Quantitative analysis of apoptosis, as determined by the Annexin V APC/7-AAD test. C Mitochondrial membrane potential was detected by JC-1 and flow cytometry. Red indicates intact mitochondrial membrane potential; green indicates damaged mitochondrial membrane potential. D Quantitative analysis of the fluorescence intensity of JC-1 (the rate of red/green). E KGN cell apoptosis was measured by TUNEL assays. Green fluorescence represents apoptotic cells, and blue fluorescence represents the nuclei of all cells. Scale bar = 20 μm. F Quantitative analysis of the TUNEL assay results. G Effect of CEFFE on the ATP levels in KGN cells. The data are represented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, compared with CTX group